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cell culture ectocervical  (ATCC)


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    ATCC cell culture ectocervical
    Cell Culture Ectocervical, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 369 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cell culture ectocervical/product/ATCC
    Average 96 stars, based on 369 article reviews
    cell culture ectocervical - by Bioz Stars, 2026-04
    96/100 stars

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    In vitro tenofovir-dp formation in PBMCs and epithelial cells

    Journal: Journal of Antimicrobial Chemotherapy

    Article Title: Single-dose pharmacokinetics of tenofovir alafenamide and its active metabolite in the mucosal tissues

    doi: 10.1093/jac/dkx064

    Figure Lengend Snippet: In vitro tenofovir-dp formation in PBMCs and epithelial cells

    Article Snippet: Cell culture methods Ectocervical (Ect1/E6E7) ATCC ® CRL-2614TM and vaginal (VK2/E6E7) ATCC ® CRL-2616TM epithelial cell lines were obtained and sub-cultured for >4 passes after the initial thaw.

    Techniques: In Vitro

    Human ectocervical cells (ECT1) were pretreated with fibronectin to block integrin-ligand interactions and then infected with MHV68; 24h after infection, MHV68 was found to be partially blocked from cells treated with fibronectin (Fibronectin) compared to cells treated with MHV68 but no fibronectin (NT)(3 independent experiments) (A). Western blot analysis showed integrin alpha 3 and beta 1 were upregulated in the pregnant cervix of infected mice (B) n=3. Antibodies blocking integrin alpha 3 (MHV68 + anti-integrin a3 ab) partially blocked MHV68 infection, while treatment with non specific IgG (MHV68 + IgG) did not block MHV68 infection as compared to cells only treated with MHV68 (MHV68 only) (Representative of 3 independent experiments with a minimum of 3 animals per group) (C). Significance is denoted by bars and is p <.05.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Viral infection of the pregnant cervix predisposes to ascending bacterial infection

    doi: 10.4049/jimmunol.1300661

    Figure Lengend Snippet: Human ectocervical cells (ECT1) were pretreated with fibronectin to block integrin-ligand interactions and then infected with MHV68; 24h after infection, MHV68 was found to be partially blocked from cells treated with fibronectin (Fibronectin) compared to cells treated with MHV68 but no fibronectin (NT)(3 independent experiments) (A). Western blot analysis showed integrin alpha 3 and beta 1 were upregulated in the pregnant cervix of infected mice (B) n=3. Antibodies blocking integrin alpha 3 (MHV68 + anti-integrin a3 ab) partially blocked MHV68 infection, while treatment with non specific IgG (MHV68 + IgG) did not block MHV68 infection as compared to cells only treated with MHV68 (MHV68 only) (Representative of 3 independent experiments with a minimum of 3 animals per group) (C). Significance is denoted by bars and is p <.05.

    Article Snippet: Cell culture Immortalized human ectocervical cells (ECT1, ATCC, CRL-2614) were cultured in keratinocyte serum free medium (17005-042, Gibco, Grand Island, NY) with bovine pituitary extract and hEGF supplementation as recommended by ATCC under 5% CO2 at 37°C.

    Techniques: Blocking Assay, Infection, Western Blot